A signal accumulation type of ion-sensitive field-effect transistor (SA-ISFET) acts as a biosensor capable of detecting changes in hydrogen ions due to enzymatic reactions. In this study, we describe an alkaline phosphatase (AP) activity detection method that uses SA-ISFET and a reagent containing pyrophosphate which acts as both substrate and buffer. The method enabled simple and cost-effective AP assays, when potential signals accumulated 10-fold. Calf intestinal AP did not require the cofactors Zn2+ and Mg2+ for the assay, whereas AP from Shewanella sp. T3-3 did. Different metal ion-dependence on the assays was explained by the structural comparison of both AP. In addition, assay application to a labeled enzyme detection system based on an immunoassay was discussed.
信号累積型イオン感応性電界効果トランジスタによるアルカリホスファターゼ活性の簡易・低コスト検出
信号累積型イオン感応性電界効果トランジスタによるアルカリホスファターゼ活性の簡易・低コスト検出
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