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信号累積型イオン感応性電界効果トランジスタによるアルカリホスファターゼ活性の簡易・低コスト検出
https://setsunan.repo.nii.ac.jp/records/1525
https://setsunan.repo.nii.ac.jp/records/15254ab8a88e-1064-4146-8ff0-38f9a5df9ed2
名前 / ファイル | ライセンス | アクション |
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信号累積型イオン感応性電界効果トランジスタによるアルカリホスファターゼ活性の簡易・低コスト検出 (1.3 MB)
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Copyright(c)2023 by Setsunan University
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2023-03-17 | |||||
タイトル | ||||||
タイトル | 信号累積型イオン感応性電界効果トランジスタによるアルカリホスファターゼ活性の簡易・低コスト検出 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Simple and cost-effective detection of alkaline phosphatase activity using a signal accumulation type of ion-sensitive field-effect transistor | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | トランジスタ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | バイオセンサ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 酵素 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ホスファターゼ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ピロリン酸 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | transistor | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | biosensor | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | enzyme | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phosphatase | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | pyrophosphate | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
佐々本, 康平
× 佐々本, 康平× 寺田, 理一郎× 西矢, 芳昭× SASAMOTO, Kohei× TERADA, Riichiro× NISHIYA, Yoshiaki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A signal accumulation type of ion-sensitive field-effect transistor (SA-ISFET) acts as a biosensor capable of detecting changes in hydrogen ions due to enzymatic reactions. In this study, we describe an alkaline phosphatase (AP) activity detection method that uses SA-ISFET and a reagent containing pyrophosphate which acts as both substrate and buffer. The method enabled simple and cost-effective AP assays, when potential signals accumulated 10-fold. Calf intestinal AP did not require the cofactors Zn2+ and Mg2+ for the assay, whereas AP from Shewanella sp. T3-3 did. Different metal ion-dependence on the assays was explained by the structural comparison of both AP. In addition, assay application to a labeled enzyme detection system based on an immunoassay was discussed. | |||||
記事種別 | ||||||
研究論文 | ||||||
書誌情報 |
摂南大学 融合科学研究所論文集 en : Bulletin of the Transdisciplinary and Interdisciplinary Science Research Institute. Setsunan University 巻 8, 号 1, p. 31-43, 発行日 2023-02 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2432-5031 |