@article{oai:setsunan.repo.nii.ac.jp:00001385,
 author = {平松, 優一 and 宮本, 敦希 and 井尻, 貴之 and HIRAMATSU, Yuichi and MIYAMOTO, Atsuki and IJIRI, Takashi},
 issue = {1},
 journal = {摂南大学　融合科学研究所論文集, Bulletin of the Transdisciplinary and Interdisciplinary Science Research Institute, Setsunan University},
 month = {Mar},
 note = {Mammalian sperm are produced in the testis and undergo biochemical and physiological modifications during passage through the epididymis and female reproductive organs, resulting in the acquisition of motility and the ability to fertilize an egg. These changes include hyperactivation that is characterized by asymmetric flagellar movement with a high amplitude, increasing in the fluidity of the sperm cell membrane, tyrosine phosphorylation of sperm proteins, and exocytosis that occurs in the acrosome. Adenosine monophosphate (AMP)-activated protein kinase (AMPK) is a sensor of cellular energy. When AMPK is activated by phosphorylation, it switches on the pathways that produce ATP and also switches off the pathways that consume ATP. Therefore, to understand the mechanisms of energy metabolism in sperm, we examined the effects of two AMPK activators, metformin and AICAR, on mouse sperm. In this study, the phosphorylation of AMPK was confirmed by immunoblot analysis, and then the total ATP level was measured using the CellTiter-GloR assay. Our results suggest that the net amount of ATP was increased by adding the AMPK activators to non-capacitated, capacitated, and acrosome-reacted sperm in the mouse. Especially, activation of AMPK by the treatment with AICAR in mouse sperm, not only promoted the induction rate of acrosome reaction and increase in the amount of ATP, but also improved the motility. On the other hand, the activation of AMPK with metformin treatment promoted an increase in acrosome reaction and the ATP amount, but weakened sperm motility. Therefore, AICAR is more suitable than metformin as an AMPK activator for recovery of sperm function in fertility treatment.},
 pages = {28--41},
 title = {AMPK活性化剤によるマウス精子のATP量の変化},
 volume = {6},
 year = {2021},
 yomi = {ヒラマツ, ユウイチ and ミヤモト, アツキ and イジリ, タカシ}
}