@article{oai:setsunan.repo.nii.ac.jp:00000988,
 author = {青木, 駿介 and 西矢, 芳昭 and AOKI, Shunsuke and NISHIYA, Yoshiaki},
 issue = {1},
 journal = {摂南大学 融合科学研究所論文集, Bulletin of the Transdisciplinary Science Research Institute, Setsunan University},
 month = {Oct},
 note = {A direct polymerase chain reaction (PCR) method from fruitbody tissues of commercially available mushrooms was developed for experimental teaching. This method was combined with easy sampling of a mushroom and using KOD FX Neo as DNA polymerase. From four mushroom species, Enokitake (Flammulina velutipes), Bunashimeji (Hypsizygus marmoreus), Maitake (Grifola frondosa), and Mushroom (Agaricus bisporus), the ribosomal DNA fragments amplified by the direct PCR could be detected at the expected size by agarose gel electrophoresis. Especially, Maitake was most suitable as a teaching material for simple manipulation. The amplified DNAs were verified by sequencing, and were identified by database search. High school students learn by this teaching method which deepens their understanding of PCR techniques, microorganisms, and bioinformatics.},
 pages = {10--17},
 title = {生物教育実験のための市販キノコ・ダイレクトPCR法},
 volume = {2},
 year = {2016}
}