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生物教育実験のための市販キノコ・ダイレクトPCR法
https://setsunan.repo.nii.ac.jp/records/988
https://setsunan.repo.nii.ac.jp/records/9881e0d01de-ebde-477d-ad5c-fafdb9f60b4f
名前 / ファイル | ライセンス | アクション |
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生物教育実験のための市販キノコ・ダイレクトPCR法 (2.1 MB)
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Copyright (c) 2017 by Setsunan University
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-03-31 | |||||
タイトル | ||||||
タイトル | 生物教育実験のための市販キノコ・ダイレクトPCR法 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | A Direct PCR Method from Commercially Available Mushrooms for Experimental Teaching | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ダイレクトPCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | キノコ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 生物教育 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 教材 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 高校 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | direct PCR | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | mushroom | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | biological education | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | teaching material | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | high school | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
青木, 駿介
× 青木, 駿介× 西矢, 芳昭× AOKI, Shunsuke× NISHIYA, Yoshiaki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A direct polymerase chain reaction (PCR) method from fruitbody tissues of commercially available mushrooms was developed for experimental teaching. This method was combined with easy sampling of a mushroom and using KOD FX Neo as DNA polymerase. From four mushroom species, Enokitake (Flammulina velutipes), Bunashimeji (Hypsizygus marmoreus), Maitake (Grifola frondosa), and Mushroom (Agaricus bisporus), the ribosomal DNA fragments amplified by the direct PCR could be detected at the expected size by agarose gel electrophoresis. Especially, Maitake was most suitable as a teaching material for simple manipulation. The amplified DNAs were verified by sequencing, and were identified by database search. High school students learn by this teaching method which deepens their understanding of PCR techniques, microorganisms, and bioinformatics. | |||||
記事種別 | ||||||
研究論文 | ||||||
書誌情報 |
摂南大学 融合科学研究所論文集 en : Bulletin of the Transdisciplinary Science Research Institute, Setsunan University 巻 2, 号 1, p. 10-17, 発行日 2016-10-31 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2432-5031 |